Hotspot

ndhJ

LSC · 51891-52367 · 477 bp

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Pi 0.00321
MarkerSeek score 43.90
Primer available yes
Label ndhJ

Feature Metadata

label_name
ndhJ
feature_type
gene
region
LSC
length_bp
477
variable_sites
9
pi
0.00321
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ_p1 TGAGACTTCAATTCCTCCGT AGGCAGGAGGATTCATTTGT 45.0 45.0 56.2 56.8 684-689 bp 0.971429 56.1
ndhJ_p2 TGAGACTTCAATTCCTCCGTT AGGCAGGAGGATTCATTTGT 42.9 45.0 57.0 56.8 684-689 bp 0.971429 54.3
ndhJ_p3 TGAGACTTCAATTCCTCCGTTT AGGCAGGAGGATTCATTTGT 40.9 45.0 57.6 56.8 684-689 bp 1.0 53.1
ndhJ_p4 ATGAGACTTCAATTCCTCCGT AGGCAGGAGGATTCATTTGT 42.9 45.0 56.7 56.8 685-690 bp 0.971429 52.5
ndhJ_p5 AGACTTCAATTCCTCCGTTTGA AGGCAGGAGGATTCATTTGT 40.9 45.0 57.6 56.8 682-687 bp 0.971429 51.8