Hotspot

ndhE-ndhG

SSC · 123063-123339 · 277 bp

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Pi 0.0183
MarkerSeek score 59.90
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
277
variable_sites
26
pi
0.0183
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 CATCAAGGCCCGAACCATAT AATAGGTGCAATTGCCGTGG 50.0 50.0 57.7 59.2 395-422 bp 1.0 75.5
ndhE-ndhG_p2 AAGGCCCGAACCATATTTCG AATAGGTGCAATTGCCGTGG 50.0 50.0 58.3 59.2 391-418 bp 1.0 75.1
ndhE-ndhG_p3 CGAACCATATTTCGGCTCGT AATAGGTGCAATTGCCGTGG 50.0 50.0 58.4 59.2 385-412 bp 1.0 74.4
ndhE-ndhG_p4 CATCAAGGCCCGAACCATAT ATAGGTGCAATTGCCGTGG 50.0 52.6 57.7 58.5 394-421 bp 1.0 72.9
ndhE-ndhG_p5 CATCAAGGCCCGAACCATAT TAATAGGTGCAATTGCCGTG 50.0 45.0 57.7 56.5 396-423 bp 1.0 72.8