Hotspot

rpl14-rpl16

LSC · 85014-85159 · 146 bp

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Pi 0.0177
MarkerSeek score 58.90
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
146
variable_sites
12
pi
0.0177
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.92
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 CACCGCTATTGTCTGCTACA CAATAGCGGCGTCCAAAATG 50.0 50.0 57.7 58.2 225-250 bp 1.0 84.3
rpl14-rpl16_p2 CACCGCTATTGTCTGCTACA TCAATAGCGGCGTCCAAAAT 50.0 45.0 57.7 58.3 226-251 bp 1.0 83.7
rpl14-rpl16_p3 CACCGCTATTGTCTGCTACA ATTTCAATAGCGGCGTCCAA 50.0 45.0 57.7 58.3 229-254 bp 1.0 83.6
rpl14-rpl16_p4 ACACATCAATTCTCGGGCAC CAATAGCGGCGTCCAAAATG 50.0 50.0 58.5 58.2 242-267 bp 1.0 82.0
rpl14-rpl16_p5 CACCGCTATTGTCTGCTACA TTCAATAGCGGCGTCCAAAA 50.0 45.0 57.7 58.5 227-252 bp 1.0 81.9