Hotspot

psbM-trnD_guc

LSC · 31401-32604 · 1204 bp

Back to Citrus

Pi 0.00764
MarkerSeek score 56.10
Primer available yes
Label psbM-trnD_guc

Feature Metadata

label_name
psbM-trnD_guc
feature_type
igs
region
LSC
length_bp
1204
variable_sites
50
pi
0.00764
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD_guc_p1 ACGCAGTAGGAACTAGAACG TTCAATTGGTCAGAGCACCG 50.0 50.0 57.1 58.5 1299-1361 bp 1.0 74.4
psbM-trnD_guc_p2 AGCAAAAACGCAGTAGGAAC TTCAATTGGTCAGAGCACCG 45.0 50.0 56.9 58.5 1306-1368 bp 1.0 73.3
psbM-trnD_guc_p3 GCAAAAACGCAGTAGGAACT TTCAATTGGTCAGAGCACCG 45.0 50.0 56.9 58.5 1305-1367 bp 1.0 73.3
psbM-trnD_guc_p4 CGCAGTAGGAACTAGAACGA TTCAATTGGTCAGAGCACCG 50.0 50.0 56.8 58.5 1298-1360 bp 1.0 72.2
psbM-trnD_guc_p5 AACGCAGTAGGAACTAGAACG TTCAATTGGTCAGAGCACCG 47.6 50.0 57.8 58.5 1300-1362 bp 1.0 72.1