Hotspot

rps16-trnQ_uug

LSC · 6150-7441 · 1292 bp

Back to Citrus

Pi 0.00726
MarkerSeek score 55.90
Primer available yes
Label rps16-trnQ_uug

Feature Metadata

label_name
rps16-trnQ_uug
feature_type
igs
region
LSC
length_bp
1292
variable_sites
59
pi
0.00726
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_uug_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 878-1428 bp 1.0 86.6
rps16-trnQ_uug_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 876-1426 bp 1.0 86.6
rps16-trnQ_uug_p3 CAGCGGATCATGTCCTTCAA CGAAATTGAAATGGGGCGTC 50.0 50.0 58.0 58.1 937-1487 bp 1.0 86.4
rps16-trnQ_uug_p4 CAGCGGATCATGTCCTTCAA GAAATTGAAATGGGGCGTCG 50.0 50.0 58.0 58.1 936-1486 bp 1.0 86.4
rps16-trnQ_uug_p5 GCGGATCATGTCCTTCAAGT GAAATTGAAATGGGGCGTCG 50.0 50.0 58.0 58.1 934-1484 bp 1.0 86.4