Hotspot

ycf1

SSC · 126504-132041 · 5538 bp

Back to Citrullus

Pi 0.00921

MarkerSeek score 45.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 100
pi: 0.00921
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAAATACCTATGGTTTGAGACA	TTGTCTCCTGCTTAGACTGA	34.8	45.0	55.3	55.5	205 bp	1.0	43.1
ycf1_p2	TGAAATACCTATGGTTTGAGACA	TGTCTCCTGCTTAGACTGAT	34.8	45.0	55.3	55.3	204 bp	1.0	43.1
ycf1_p3	TGAAATACCTATGGTTTGAGACA	ATTGTCTCCTGCTTAGACTGA	34.8	42.9	55.3	56.1	206 bp	1.0	43.1
ycf1_p4	TGAAATACCTATGGTTTGAGACA	TTGTCTCCTGCTTAGACTGAT	34.8	42.9	55.3	56.1	205 bp	1.0	43.1
ycf1_p5	TGAAATACCTATGGTTTGAGACAT	TTGTCTCCTGCTTAGACTGA	33.3	45.0	55.8	55.5	205 bp	1.0	43.1