Hotspot

atpB

LSC · 55233-56729 · 1497 bp

Back to Citrullus

Pi 0.00412

MarkerSeek score 60.50

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 12
pi: 0.00412
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CAATAGGTGCGTGATCTGGT	GTGCGAACTCCCATTTCATT	50.0	45.0	57.7	56.7	1734 bp	1.0	67.6
atpB_p2	CAATAGGTGCGTGATCTGGT	CCGATCAACTTGCTTTTCCA	50.0	45.0	57.7	56.6	1700 bp	1.0	67.1
atpB_p3	CAATAGGTGCGTGATCTGGT	ACCGATCAACTTGCTTTTCC	50.0	45.0	57.7	56.6	1701 bp	1.0	67.0
atpB_p4	CAATAGGTGCGTGATCTGGT	ACCGATCAACTTGCTTTTCCA	50.0	42.9	57.7	58.4	1701 bp	1.0	66.7
atpB_p5	TTCGATTCGGAGTCAGGACA	GTGCGAACTCCCATTTCATT	50.0	45.0	58.5	56.7	1650 bp	1.0	66.5