Hotspot

ndhF-rpl32

SSC · 115203-115855 · 653 bp

Back to Citrullus

Pi 0.0108
MarkerSeek score 55.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
653
variable_sites
14
pi
0.0108
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGGAAGTGAGATGAACGGT TGATAGAGAAGTGCGCTTTT 45.0 40.0 56.8 55.1 732-738 bp 1.0 49.4
ndhF-rpl32_p2 TTGGAAGTGAGATGAACGGT TTGATAGAGAAGTGCGCTTT 45.0 40.0 56.8 55.1 733-739 bp 1.0 49.4
ndhF-rpl32_p3 TTGGAAGTGAGATGAACGGT TTTGATAGAGAAGTGCGCTT 45.0 40.0 56.8 55.1 734-740 bp 1.0 49.4
ndhF-rpl32_p4 TTGGAAGTGAGATGAACGGT TTTTGATAGAGAAGTGCGCT 45.0 40.0 56.8 55.1 735-741 bp 1.0 49.4
ndhF-rpl32_p5 TTGGAAGTGAGATGAACGGT TGATAGAGAAGTGCGCTTTTT 45.0 38.1 56.8 55.9 732-738 bp 1.0 47.8