Hotspot

petG-trnW-CCA

LSC · 68603-68718 · 116 bp

Back to Citrullus

Pi 0.0388

MarkerSeek score 63.30

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 9
pi: 0.0388
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GGGGATCAGTTGGACCTTTG	CAAAACCCGATGTCGTAGGT	55.0	50.0	58.2	57.9	176 bp	1.0	82.1
petG-trnW-CCA_p2	GGGGATCAGTTGGACCTTTG	CCCGATGTCGTAGGTTCAAA	55.0	50.0	58.2	57.6	171 bp	1.0	79.9
petG-trnW-CCA_p3	GGGGATCAGTTGGACCTTTG	TCCAAAACCCGATGTCGTAG	55.0	50.0	58.2	57.6	178 bp	1.0	79.8
petG-trnW-CCA_p4	GGGGATCAGTTGGACCTTTG	ACGCTCTTAGTTCAGTTCGG	55.0	50.0	58.2	57.7	211 bp	1.0	79.3
petG-trnW-CCA_p5	GGGGATCAGTTGGACCTTTG	CGCTCTTAGTTCAGTTCGGT	55.0	50.0	58.2	57.7	210 bp	1.0	79.3