Hotspot

trnF-GAA-ndhJ

LSC · 50631-51333 · 703 bp

Back to Citrullus

Pi 0.0123

MarkerSeek score 57.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 17
pi: 0.0123
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGTAAGGATTATATTGCCCCCA	50.0	45.5	58.1	57.7	783-818 bp	1.0	63.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ACGTAAGGATTATATTGCCCCC	50.0	45.5	58.1	57.7	784-819 bp	1.0	63.4
trnF-GAA-ndhJ_p3	AATCCTCGTGTCACCAGTTC	CGTAAGGATTATATTGCCCCCA	50.0	45.5	57.5	57.7	786-821 bp	1.0	60.2
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	ACGTAAGGATTATATTGCCCCC	50.0	45.5	57.5	57.7	787-822 bp	1.0	60.2
trnF-GAA-ndhJ_p5	CCGGGATAGCTCAGTTGGTA	CGTAAGGATTATATTGCCCCCA	55.0	45.5	58.6	57.7	821-856 bp	1.0	59.0