Hotspot

trnE-TTC-trnT-GGT

LSC · 32651-33597 · 947 bp

Back to Citrullus

Pi 0.00862

MarkerSeek score 64.00

Primer available yes

Label trnE-TTC-trnT-GGT

Feature Metadata

label_name: trnE-TTC-trnT-GGT
feature_type: igs
region: LSC
length_bp: 947
variable_sites: 16
pi: 0.00862
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-trnT-GGT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1088-1112 bp	1.0	81.9
trnE-TTC-trnT-GGT_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	1067-1091 bp	1.0	81.5
trnE-TTC-trnT-GGT_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	1064-1088 bp	1.0	81.4
trnE-TTC-trnT-GGT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1050-1074 bp	1.0	81.2
trnE-TTC-trnT-GGT_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1029-1053 bp	1.0	81.2