Hotspot

psbM-trnD-GTC

LSC · 30938-32044 · 1107 bp

Back to Citrullus

Pi 0.00843

MarkerSeek score 61.40

Primer available yes

Label psbM-trnD-GTC

Feature Metadata

label_name: psbM-trnD-GTC
feature_type: igs
region: LSC
length_bp: 1107
variable_sites: 18
pi: 0.00843
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GTC_p1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1113-1232 bp	1.0	73.2
psbM-trnD-GTC_p2	AGGCGGTAGGAACTAGAATGA	GTTCAATTGGTCAGAGCACC	47.6	50.0	58.0	57.0	1114-1233 bp	1.0	69.0
psbM-trnD-GTC_p3	GGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1112-1231 bp	1.0	68.9
psbM-trnD-GTC_p4	AGGCGGTAGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1113-1232 bp	1.0	68.8
psbM-trnD-GTC_p5	AGGCGGTAGGAACTAGAATGA	TCAATTGGTCAGAGCACCG	47.6	52.6	58.0	57.8	1112-1231 bp	1.0	67.1