Hotspot

petN-psbM

LSC · 29958-30832 · 875 bp

Back to Citrullus

Pi 0.00952

MarkerSeek score 65.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 16
pi: 0.00952
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	TCATTCTAGTTCCTACCGCCT	50.0	47.6	57.6	58.0	962-969 bp	1.0	70.3
petN-psbM_p2	CCCTCGTAGTATGGGGAAGA	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.6	58.0	972-979 bp	1.0	70.1
petN-psbM_p3	TCCCTCGTAGTATGGGGAAG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.6	58.0	973-980 bp	1.0	70.1
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	976-983 bp	1.0	67.2
petN-psbM_p5	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	986-993 bp	1.0	67.0