Hotspot

atpA-atpF

LSC · 12912-12971 · 60 bp

Back to Citrullus

Pi 0.0417

MarkerSeek score 61.80

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 5
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	ATTGGCATGTTTGGGACGAT	55.0	45.0	58.1	58.1	212-213 bp	1.0	79.9
atpA-atpF_p2	TTGCTCAATACGTTCACGGA	ATTGGCATGTTTGGGACGAT	45.0	45.0	57.6	58.1	156-157 bp	1.0	77.8
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	TATTGGCATGTTTGGGACGA	55.0	45.0	58.1	57.5	213-214 bp	1.0	77.1
atpA-atpF_p4	TGCTCAATACGTTCACGGAT	ATTGGCATGTTTGGGACGAT	45.0	45.0	57.3	58.1	155-156 bp	1.0	76.1
atpA-atpF_p5	CGTCGCCTACTTGAAGTACA	ATTGGCATGTTTGGGACGAT	50.0	45.0	57.4	58.1	211-212 bp	1.0	75.7