Hotspot

trnG-TCC-trnR-TCT

LSC · 10481-10758 · 278 bp

Back to Citrullus

Pi 0.0296

MarkerSeek score 76.90

Primer available yes

Label trnG-TCC-trnR-TCT

Feature Metadata

label_name: trnG-TCC-trnR-TCT
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 16
pi: 0.0296
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-TCC-trnR-TCT_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	328-358 bp	1.0	71.0
trnG-TCC-trnR-TCT_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	331-361 bp	1.0	70.8
trnG-TCC-trnR-TCT_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	334-364 bp	1.0	70.3
trnG-TCC-trnR-TCT_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	337-367 bp	1.0	70.0
trnG-TCC-trnR-TCT_p5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.3	56.9	398-428 bp	1.0	68.6