Hotspot

trnS-GCT-trnG-TCC

LSC · 8944-9716 · 773 bp

Back to Citrullus

Pi 0.0106

MarkerSeek score 64.90

Primer available yes

Label trnS-GCT-trnG-TCC

Feature Metadata

label_name: trnS-GCT-trnG-TCC
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 15
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnG-TCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	842-872 bp	1.0	72.4
trnS-GCT-trnG-TCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	841-871 bp	1.0	72.4
trnS-GCT-trnG-TCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	842-872 bp	1.0	72.3
trnS-GCT-trnG-TCC_p4	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	841-871 bp	1.0	72.3
trnS-GCT-trnG-TCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.2	58.2	896-926 bp	1.0	71.1