Hotspot

trnK-TTT-trnQ-TTG

LSC · 4197-7621 · 3425 bp

Back to Citrullus

Pi 0.0111

MarkerSeek score 55.00

Primer available yes

Label trnK-TTT-trnQ-TTG

Feature Metadata

label_name: trnK-TTT-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 3425
variable_sites: 73
pi: 0.0111
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-TTT-trnQ-TTG_p1	GATTAAACGATTGTTCCTACCT	AATGGTGTGTGAAACCCGAG	36.4	50.0	53.9	58.4	175-181 bp	1.0	44.1
trnK-TTT-trnQ-TTG_p2	ATTAAACGATTGTTCCTACCT	AAATGGTGTGTGAAACCCGA	33.3	45.0	52.5	57.9	175-181 bp	1.0	44.1
trnK-TTT-trnQ-TTG_p3	GATTAAACGATTGTTCCTACCT	GGTGTGTGAAACCCGAGTAT	36.4	50.0	53.9	57.5	172-178 bp	1.0	44.1
trnK-TTT-trnQ-TTG_p4	GATTAAACGATTGTTCCTACCT	AAATGGTGTGTGAAACCCGA	36.4	45.0	53.9	57.9	176-182 bp	1.0	44.0
trnK-TTT-trnQ-TTG_p5	GATTAAACGATTGTTCCTACC	AAATGGTGTGTGAAACCCGA	38.1	45.0	52.4	57.9	176-182 bp	1.0	44.0