Hotspot

ndhH-rps15

SSC · 127314-127414 · 101 bp

Back to Citropsis

Pi 0.033
MarkerSeek score 50.10
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
101
variable_sites
5
pi
0.033
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 GGTGGTGAGGTCCCATATTG CGTTGAATATCCGGGAGTCA 55.0 50.0 57.7 57.1 197-201 bp 1.0 72.2
ndhH-rps15_p2 GGTGGTGAGGTCCCATATTG ACGTTGAATATCCGGGAGTC 55.0 50.0 57.7 57.1 198-202 bp 1.0 72.1
ndhH-rps15_p3 AGACGAAGAACACCATGCAT CGTTGAATATCCGGGAGTCA 45.0 50.0 57.5 57.1 222-226 bp 1.0 70.9
ndhH-rps15_p4 AGACGAAGAACACCATGCAT ACGTTGAATATCCGGGAGTC 45.0 50.0 57.5 57.1 223-227 bp 1.0 70.9
ndhH-rps15_p5 GGTGGTGAGGTCCCATATTG CGTTGAATATCCGGGAGTCAA 55.0 47.6 57.7 57.8 197-201 bp 1.0 69.8