Hotspot

ndhE-ndhG

SSC · 122117-122400 · 284 bp

Back to Citropsis

Pi 0.017

MarkerSeek score 54.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 284
variable_sites: 7
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCAAGACACATCAAGGCCC	AATAGGTGCAATTGCCGTGG	50.0	50.0	58.1	59.2	410-420 bp	1.0	73.1
ndhE-ndhG_p2	CATCAAGGCCCGAACCATAT	AATAGGTGCAATTGCCGTGG	50.0	50.0	57.7	59.2	401-411 bp	1.0	71.7
ndhE-ndhG_p3	AAGGCCCGAACCATATTTCG	AATAGGTGCAATTGCCGTGG	50.0	50.0	58.3	59.2	397-407 bp	1.0	71.3
ndhE-ndhG_p4	TTCAAGACACATCAAGGCCC	ATAGGTGCAATTGCCGTGG	50.0	52.6	58.1	58.5	409-419 bp	1.0	70.5
ndhE-ndhG_p5	TTCAAGACACATCAAGGCCC	TAATAGGTGCAATTGCCGTG	50.0	45.0	58.1	56.5	411-421 bp	1.0	70.5