Hotspot

psbE-petL

LSC · 67407-68775 · 1369 bp

Back to Citropsis

Pi 0.00538

MarkerSeek score 50.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1369
variable_sites: 11
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1515-1516 bp	1.0	71.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1514-1515 bp	1.0	71.3
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.8	56.9	1438-1439 bp	1.0	70.5
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1437-1438 bp	1.0	70.5
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCCGCTAGTAGAAAACCAA	45.0	45.0	58.2	56.9	1523-1524 bp	1.0	70.4