Hotspot

petA-psbJ

LSC · 65527-66621 · 1095 bp

Back to Citropsis

Pi 0.011

MarkerSeek score 53.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 18
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.0	58.1	1234-1237 bp	1.0	80.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCCGGATTAGGTTCGTCCTT	50.0	50.0	58.0	58.1	1235-1238 bp	1.0	79.9
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.2	58.1	1231-1234 bp	1.0	79.4
petA-psbJ_p4	GAGAAGGTGCAATTGTCCGA	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.2	58.1	1148-1151 bp	1.0	79.4
petA-psbJ_p5	GCATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1199-1202 bp	1.0	79.1