Hotspot

psaA-pafI

LSC · 44526-45242 · 717 bp

Back to Citropsis

Pi 0.00766

MarkerSeek score 53.80

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 8
pi: 0.00766
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TGGTTCTTGAGAAATGCCCC	ATCAAGCTGCCGAGTATTGG	50.0	50.0	58.1	58.0	900-921 bp	1.0	81.6
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	874-895 bp	1.0	81.4
psaA-pafI_p3	TGGTTCTTGAGAAATGCCCC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.1	58.1	842-863 bp	1.0	81.3
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	816-837 bp	1.0	81.1
psaA-pafI_p5	TGGTTCTTGAGAAATGCCCC	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.1	57.8	833-854 bp	1.0	80.7