Hotspot

psbC-trnS-UGA

LSC · 37696-37942 · 247 bp

Back to Citropsis

Pi 0.027

MarkerSeek score 57.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 10
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	393 bp	1.0	80.5
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	339 bp	1.0	79.6
psbC-trnS-UGA_p3	CGTAGGTCACTTATGGCACG	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.4	58.1	433 bp	1.0	77.8
psbC-trnS-UGA_p4	GCAGCAGGGTTTGAAAAAGG	TGGTTGATAGCTCCGGTCTT	50.0	50.0	58.1	58.4	394 bp	1.0	77.5
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	TAGCTCCGGTCTTGAAAACC	50.0	50.0	58.1	57.5	387 bp	1.0	77.3