Hotspot

atpH-atpI

LSC · 14507-15487 · 981 bp

Back to Citropsis

Pi 0.00612

MarkerSeek score 50.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 981
variable_sites: 9
pi: 0.00612
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1115-1369 bp	1.0	79.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1068-1322 bp	1.0	79.2
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	1171-1425 bp	1.0	78.6
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1124-1378 bp	1.0	78.6
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	1153-1407 bp	1.0	78.5