Hotspot

trnR-UCU-atpA

LSC · 10696-10890 · 195 bp

Back to Citropsis

Pi 0.0274

MarkerSeek score 51.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 195
variable_sites: 8
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AATGGAATGAAAGGCGTCCA	GAAGCCCTTTTGCAAGAAGC	45.0	50.0	57.8	58.2	346-377 bp	1.0	78.3
trnR-UCU-atpA_p2	TGGAATGAAAGGCGTCCATT	GAAGCCCTTTTGCAAGAAGC	45.0	50.0	57.8	58.2	344-375 bp	1.0	78.3
trnR-UCU-atpA_p3	AATGGAATGAAAGGCGTCCA	AGCCCTTTTGCAAGAAGCTA	45.0	45.0	57.8	57.7	344-375 bp	1.0	77.7
trnR-UCU-atpA_p4	TGGAATGAAAGGCGTCCATT	AGCCCTTTTGCAAGAAGCTA	45.0	45.0	57.8	57.7	342-373 bp	1.0	77.7
trnR-UCU-atpA_p5	ATGGAATGAAAGGCGTCCAT	GAAGCCCTTTTGCAAGAAGC	45.0	50.0	57.6	58.2	345-376 bp	1.0	76.5