Hotspot

ycf1

SSC · 126444-132191 · 5748 bp

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Pi 0.0115

MarkerSeek score 44.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5748
variable_sites: 66
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAACCCTATCTATTTTCTGTTCA	ACCAAAAATATACGATCCTTTATT	33.3	25.0	55.5	52.5	189-192 bp	1.0	47.6
ycf1_p2	TCAACCCTATCTATTTTCTGTTCA	TTTACCAAAAATATACGATCCTTT	33.3	25.0	55.5	52.8	192-195 bp	1.0	47.4
ycf1_p3	TCAACCCTATCTATTTTCTGTTCA	ATTTACCAAAAATATACGATCCTT	33.3	25.0	55.5	52.5	193-196 bp	1.0	47.4
ycf1_p4	TCAACCCTATCTATTTTCTGTTCAA	TTTACCAAAAATATACGATCCTTT	32.0	25.0	56.2	52.8	192-195 bp	1.0	47.4
ycf1_p5	TTCAACCCTATCTATTTTCTGTTCA	TTTACCAAAAATATACGATCCTTT	32.0	25.0	56.2	52.8	193-196 bp	1.0	47.4