Hotspot

rpl20

LSC · 70670-71056 · 387 bp

Back to Cistanthe

Pi 0.0233

MarkerSeek score 55.60

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 9
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	GGAGAATAAACTCCGGGGAAC	AATAGACCAGGAAGGGAAAA	52.4	40.0	58.1	53.5	490 bp	1.0	41.5
rpl20_p2	GGAGAATAAACTCCGGGGAAC	ATAGACCAGGAAGGGAAAAA	52.4	40.0	58.1	53.5	489 bp	1.0	41.5
rpl20_p3	GGAGAATAAACTCCGGGGAAC	AATAGACCAGGAAGGGAAAAA	52.4	38.1	58.1	54.4	490 bp	1.0	41.5
rpl20_p4	ACTCTATCCTCCCGGAGAAT	AATAGACCAGGAAGGGAAAA	50.0	40.0	56.6	53.5	503 bp	1.0	41.4
rpl20_p5	ACTCTATCCTCCCGGAGAAT	ATAGACCAGGAAGGGAAAAA	50.0	40.0	56.6	53.5	502 bp	1.0	41.4