Hotspot

rrn23-trnA-UGC

IRa · 136895-137043 · 149 bp

Back to Cistanthe

Pi 0.0738

MarkerSeek score 69.90

Primer available yes

Label rrn23-trnA-UGC

Feature Metadata

label_name: rrn23-trnA-UGC
feature_type: igs
region: IRa
length_bp: 149
variable_sites: 11
pi: 0.0738
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-trnA-UGC_p1	ATTCGGGAATCTCCGGATCT	GGCTGTCAAGAACGTAGAGG	50.0	55.0	58.0	58.0	969-1003 bp	1.0	80.9
rrn23-trnA-UGC_p2	ATTCGGGAATCTCCGGATCT	GGGCTGTCAAGAACGTAGAG	50.0	55.0	58.0	58.0	970-1004 bp	1.0	80.9
rrn23-trnA-UGC_p3	ATGTTTCAGTTCGCCAGGTT	GGCTGTCAAGAACGTAGAGG	45.0	55.0	58.0	58.0	1036-1070 bp	1.0	80.8
rrn23-trnA-UGC_p4	AGTGATGCTTTCGGCTACTG	GGCTGTCAAGAACGTAGAGG	50.0	55.0	58.0	58.0	1215-1249 bp	1.0	80.7
rrn23-trnA-UGC_p5	AGTGATGCTTTCGGCTACTG	GGGCTGTCAAGAACGTAGAG	50.0	55.0	58.0	58.0	1216-1250 bp	1.0	80.7