Hotspot

psbC-trnS-UGA

LSC · 35816-36069 · 254 bp

Back to Cistanthe

Pi 0.0395

MarkerSeek score 75.60

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 10
pi: 0.0395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCGATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.3	58.0	376 bp	1.0	80.1
psbC-trnS-UGA_p2	CCTGTTCTTTCGATGACCCC	GTTGGAGAGATGGCTGAGTG	55.0	55.0	58.3	58.0	380 bp	1.0	80.1
psbC-trnS-UGA_p3	CCTGTTCTTTCGATGACCCC	GCTGAGTGGTTGATAGCTCC	55.0	55.0	58.3	58.1	368 bp	1.0	79.9
psbC-trnS-UGA_p4	CCTGTTCTTTCGATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.3	58.1	369 bp	1.0	79.9
psbC-trnS-UGA_p5	CCTGTTCTTTCGATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	361 bp	1.0	79.9