Hotspot

trnD-GUC-trnY-GUA

LSC · 30688-31060 · 373 bp

Back to Cistanthe

Pi 0.0188

MarkerSeek score 53.50

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 7
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	443-448 bp	1.0	75.5
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	442-447 bp	1.0	75.5
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	464-469 bp	1.0	74.6
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	463-468 bp	1.0	74.4
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	497-502 bp	1.0	74.0