Hotspot

atpA-atpF

LSC · 11444-11511 · 68 bp

Back to Cistanthe

Pi 0.0294
MarkerSeek score 69.40
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
68
variable_sites
2
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CAATGCCGTCACCTACTTGA GCATGTTTGGTGCCATGAAA 50.0 45.0 57.9 57.8 221-223 bp 1.0 78.6
atpA-atpF_p2 ATGCCGTCACCTACTTGAAG GCATGTTTGGTGCCATGAAA 50.0 45.0 57.6 57.8 219-221 bp 1.0 76.3
atpA-atpF_p3 TGCTAATTTCGTCTGCTCGA GCATGTTTGGTGCCATGAAA 45.0 45.0 57.4 57.8 134-136 bp 1.0 74.7
atpA-atpF_p4 AATGCCGTCACCTACTTGAA GCATGTTTGGTGCCATGAAA 45.0 45.0 57.1 57.8 220-222 bp 1.0 72.6
atpA-atpF_p5 TGCCGTCACCTACTTGAAGT GCATGTTTGGTGCCATGAAA 50.0 45.0 59.0 57.8 218-220 bp 1.0 71.8