Hotspot

psbK-psbI

LSC · 7325-7674 · 350 bp

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Pi 0.0289

MarkerSeek score 68.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 350
variable_sites: 10
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCTTCGCGTCCAGGATTAC	50.0	50.0	57.9	57.7	484-488 bp	1.0	79.5
psbK-psbI_p2	AAATTACCCGAAGCCTACGC	TTCTTCGCGTCCAGGATTAC	50.0	50.0	58.3	57.7	568-572 bp	1.0	76.9
psbK-psbI_p3	TGTTTGGCAAGCTGCTGTAA	TTCTTCGCGTCCAGGATTAC	45.0	50.0	58.6	57.7	485-489 bp	1.0	75.2
psbK-psbI_p4	TTGTTTGGCAAGCTGCTGTA	TTCTTCGCGTCCAGGATTAC	45.0	50.0	58.6	57.7	486-490 bp	1.0	75.2
psbK-psbI_p5	CTTTGTTTGGCAAGCTGCTG	TTCTTCGCGTCCAGGATTAC	50.0	50.0	58.8	57.7	488-492 bp	1.0	73.7