Hotspot

rpl32-trnL-UAG

SSC · 117182-118300 · 1119 bp

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Pi 0.0425
MarkerSeek score 77.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1119
variable_sites
98
pi
0.0425
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTGGAAAAGGAAAGGACGGC AAGCACTGCTTCCTAAGAGC 50.0 50.0 58.7 57.9 1301-1357 bp 0.6 63.1
rpl32-trnL-UAG_p2 TTGGAAAAGGAAAGGACGGC GCTCAAGCACTGCTTCCTAA 50.0 50.0 58.7 58.2 1305-1361 bp 0.6 62.5
rpl32-trnL-UAG_p3 TTGGAAAAGGAAAGGACGGC CACTTGGCCCGGTTATTGTA 50.0 50.0 58.7 57.9 1778-1844 bp 0.6 62.4
rpl32-trnL-UAG_p4 TTGGAAAAGGAAAGGACGGC ACTTGGCCCGGTTATTGTAC 50.0 50.0 58.7 57.9 1777-1843 bp 0.6 62.4
rpl32-trnL-UAG_p5 TTGGAAAAGGAAAGGACGGC GCTTCCTAAGAGCAACGTGT 50.0 50.0 58.7 58.2 1294-1350 bp 0.6 62.3