Hotspot

ndhF-rpl32

SSC · 115683-116992 · 1310 bp

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Pi 0.0411
MarkerSeek score 81.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1310
variable_sites
107
pi
0.0411
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGGAAGTGGAAGGAAAGGT TCCAAAAATTTTTACGAATACGCT 45.0 29.2 56.6 56.2 1303-1509 bp 1.0 50.1
ndhF-rpl32_p2 TTGGAAGTGGAAGGAAAGGT TCCAAAAATTTTTACGAATACGC 45.0 30.4 56.6 54.8 1303-1509 bp 1.0 50.1
ndhF-rpl32_p3 TTGGAAGTGGAAGGAAAGGT TCCAAAAATTTTTACGAATACGCTT 45.0 28.0 56.6 56.8 1303-1509 bp 1.0 50.1
ndhF-rpl32_p4 TTGGAAGTGGAAGGAAAGGT CCAAAAATTTTTACGAATACGCT 45.0 30.4 56.6 54.8 1302-1508 bp 1.0 50.1
ndhF-rpl32_p5 TTGGAAGTGGAAGGAAAGGT TTCCAAAAATTTTTACGAATACGCT 45.0 28.0 56.6 56.8 1304-1510 bp 1.0 50.0