Hotspot

psaJ-rpl33

LSC · 70321-70799 · 479 bp

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Pi 0.042
MarkerSeek score 78.30
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
479
variable_sites
44
pi
0.042
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GGGAATGCTCGGGAGTAAAG GGTCGACTCGGTTCTTTCAA 55.0 50.0 58.0 57.9 1418-1437 bp 0.8 75.3
psaJ-rpl33_p2 TGCGCTTACATTCCCCTTTT CTGTTCCTTGGAAGGGTGAC 45.0 55.0 58.1 58.1 742-748 bp 0.4 56.9
psaJ-rpl33_p3 GGGAATGCTCGGGAGTAAAG CTGTTCCTTGGAAGGGTGAC 55.0 55.0 58.0 58.1 1027-1033 bp 0.4 56.8
psaJ-rpl33_p4 ACCCGTGACATTTTGTACCC CTGTTCCTTGGAAGGGTGAC 50.0 55.0 58.1 58.1 1334 bp 0.4 56.4
psaJ-rpl33_p5 CCCGTGACATTTTGTACCCA CTGTTCCTTGGAAGGGTGAC 50.0 55.0 58.1 58.1 1333 bp 0.4 56.4