Hotspot

psbZ-trnfM-CAU

LSC · 38059-38820 · 762 bp

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Pi 0.0456
MarkerSeek score 76.70
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
LSC
length_bp
762
variable_sites
63
pi
0.0456
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 ATATGATACCGGGACAGGCA CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.0 58.0 1523-1736 bp 0.8 79.0
psbZ-trnfM-CAU_p2 ATATGATACCGGGACAGGCA CGGGACTTTGGGTTATCTGG 50.0 55.0 58.0 58.0 1814-2053 bp 0.8 78.2
psbZ-trnfM-CAU_p3 ATATGATACCGGGACAGGCA TCTTGATTGCCTCCACATCG 50.0 50.0 58.0 58.0 2202-2446 bp 0.8 77.4
psbZ-trnfM-CAU_p4 ATATGATACCGGGACAGGCA GGTTCTTCTATTCAGGGCGG 50.0 55.0 58.0 58.0 1578-1669 bp 0.6 68.9
psbZ-trnfM-CAU_p5 ATATGATACCGGGACAGGCA GTTCTTCTATTCAGGGCGGG 50.0 55.0 58.0 58.0 1577-1668 bp 0.6 68.9