Hotspot

psbC-trnS-UGA

LSC · 37144-37399 · 256 bp

Back to Cissus

Pi 0.0391
MarkerSeek score 74.70
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
256
variable_sites
24
pi
0.0391
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 GCAGCAGGGTTTGAAAAAGG GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.1 58.2 339-353 bp 1.0 86.0
psbC-trnS-UGA_p2 CTGCAGCAGGGTTTGAAAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.4 58.2 341-355 bp 1.0 82.2
psbC-trnS-UGA_p3 TGCAGCAGGGTTTGAAAAAG GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.4 58.2 340-354 bp 1.0 82.2
psbC-trnS-UGA_p4 TCGCGATTTTGAACCTGTTC GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.0 58.2 313-327 bp 1.0 80.0
psbC-trnS-UGA_p5 CGCGATTTTGAACCTGTTCT GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.0 58.2 312-326 bp 1.0 80.0