Hotspot

psbM-trnD-GUC

LSC · 30636-31775 · 1140 bp

Back to Cissus

Pi 0.0368
MarkerSeek score 73.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1140
variable_sites
74
pi
0.0368
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 35.0 50.0 52.0 58.5 758-1274 bp 1.0 49.3
psbM-trnD-GUC_p2 ATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 33.3 50.0 52.7 58.5 759-1275 bp 1.0 49.3
psbM-trnD-GUC_p3 TGCGAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 33.3 50.0 52.7 58.5 758-1274 bp 1.0 49.3
psbM-trnD-GUC_p4 AATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 31.8 50.0 53.6 58.5 760-1276 bp 1.0 49.3
psbM-trnD-GUC_p5 TGCGAGAATATTTACTTCCA GTTCAATTGGTCAGAGCACC 35.0 50.0 52.0 57.0 759-1275 bp 1.0 49.3