Hotspot

psbK-psbI

LSC · 8037-8203 · 167 bp

Back to Cissus

Pi 0.0459
MarkerSeek score 76.70
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
167
variable_sites
17
pi
0.0459
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CAAGCCGCTGTAAGTTTTCG TTTGATAGTTAAGGGCGGGC 50.0 50.0 58.0 58.0 451 bp 0.2 47.2
psbK-psbI_p2 GATTCGAACCTCCGGATAGC TTTGATAGTTAAGGGCGGGC 55.0 50.0 57.9 58.0 1032 bp 0.2 47.1
psbK-psbI_p3 AGCCGCTGTAAGTTTTCGAT TTTGATAGTTAAGGGCGGGC 45.0 50.0 57.9 58.0 449 bp 0.2 46.9
psbK-psbI_p4 TTTCTTTGCCAAATTGCCCG TTTGATAGTTAAGGGCGGGC 45.0 50.0 58.1 58.0 551 bp 0.2 46.6
psbK-psbI_p5 AAGCCGCTGTAAGTTTTCGA TTTGATAGTTAAGGGCGGGC 45.0 50.0 58.1 58.0 450 bp 0.2 46.6