Hotspot

rps16-trnQ-UUG

LSC · 6287-7419 · 1133 bp

Back to Cissus

Pi 0.0681
MarkerSeek score 82.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1133
variable_sites
140
pi
0.0681
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 880-1261 bp 1.0 90.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 859-1240 bp 1.0 90.3
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 870-1251 bp 1.0 89.8
rps16-trnQ-UUG_p4 ATCCACAACCGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 882-1263 bp 1.0 88.7
rps16-trnQ-UUG_p5 TCCACAACCGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.7 58.1 881-1262 bp 1.0 85.6