Hotspot

rps15

SSC · 121952-122230 · 279 bp

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Pi 0.251

MarkerSeek score 62.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 134
pi: 0.251
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.0	58.0	1062-1071 bp	0.5	60.5
rps15_p2	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	925-934 bp	0.5	60.3
rps15_p3	TCCCAACGCGTTACATAAGG	TTTCTTCTGTCCGGCATCTG	50.0	50.0	58.0	57.9	957-966 bp	0.5	60.0
rps15_p4	GCAGTGACTCCTCCAATACG	AATAATGGGAGCCGCTTGAG	55.0	50.0	58.1	58.0	1344-1353 bp	0.5	59.9
rps15_p5	AGGAGTCTGTGCCCCAATAT	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.1	58.0	1240-1249 bp	0.5	59.7