Hotspot

ndhH

SSC · 120673-121854 · 1182 bp

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Pi 0.219

MarkerSeek score 67.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 492
pi: 0.219
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGTTGTTGTATACCCGCAGA	TCAACGGTTGCTGGCTTATT	50.0	45.0	58.0	58.1	1550-1556 bp	0.5	59.7
ndhH_p2	TGCGTATTCAGGGCCAATAC	TCAACGGTTGCTGGCTTATT	50.0	45.0	58.0	58.1	1569-1575 bp	0.5	59.4
ndhH_p3	CGTTGTTGTATACCCGCAGA	TCTTCTGTCCGGCATCTGTA	50.0	50.0	58.0	58.2	2108-2117 bp	0.5	59.3
ndhH_p4	CCTGACATAAGAAGGCCGAC	TCAACGGTTGCTGGCTTATT	55.0	45.0	58.1	58.1	1814-1820 bp	0.5	59.2
ndhH_p5	GACATAAGAAGGCCGACAGG	TCAACGGTTGCTGGCTTATT	55.0	45.0	58.1	58.1	1811-1817 bp	0.5	59.2