Hotspot

ndhD

SSC · 114452-115972 · 1521 bp

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Pi 0.227

MarkerSeek score 73.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 659
pi: 0.227
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAGTGATTCACGCGGTTCT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	1899 bp	0.5	60.0
ndhD_p2	AGCAAATTCCGCAATTGTGG	TCTGCTCCAAGAACAGAGGA	45.0	50.0	57.9	58.0	2115 bp	0.5	59.4
ndhD_p3	GTAGTGATTCACGCGGTTCT	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	2121 bp	0.5	59.3
ndhD_p4	GTAGTGATTCACGCGGTTCT	TGATACCTTGGGACGGATGT	50.0	50.0	58.0	58.1	1937 bp	0.5	59.1
ndhD_p5	GTAGTGATTCACGCGGTTCT	CTCGGGGAACTCATCGATTC	50.0	55.0	58.0	57.9	2117 bp	0.5	59.0