Hotspot

ndhA

SSC · 118522-120671 · 2150 bp

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Pi 0.21

MarkerSeek score 71.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2150
variable_sites: 863
pi: 0.21
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	50.0	50.0	58.0	58.1	2721-2750 bp	0.5	59.7
ndhA_p2	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	50.0	50.0	58.0	58.1	2722-2751 bp	0.5	59.7
ndhA_p3	TGACCAATATACCTTGCGGC	TGGGAAGTCCAATGGCAAAA	50.0	45.0	58.0	57.9	2734-2763 bp	0.5	59.4
ndhA_p4	TGACCAATATACCTTGCGGC	TTAGAAATCCGCCGCTTTGA	50.0	45.0	58.0	57.9	2605-2634 bp	0.5	59.4
ndhA_p5	AAACGCTCGGATGTGATCAA	GGCAAAAAGAGGGGGATTCA	45.0	50.0	57.9	58.1	2808-2837 bp	0.5	59.2