Hotspot

ccsA

SSC · 113237-114205 · 969 bp

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Pi 0.201

MarkerSeek score 67.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 370
pi: 0.201
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	AGAACCGCGTGAATCACTAC	50.0	50.0	58.0	58.0	1296-1297 bp	0.5	60.3
ccsA_p2	TGAAATCGGTAGACACGCTG	AGAACCGCGTGAATCACTAC	50.0	50.0	58.0	58.0	1295-1296 bp	0.5	60.3
ccsA_p3	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1571-1572 bp	0.5	60.0
ccsA_p4	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1570-1571 bp	0.5	60.0
ccsA_p5	GTGAAATCGGTAGACACGCT	TAGCTTTTCGATGGCTTCCC	50.0	50.0	58.0	58.0	1794-1795 bp	0.5	59.9