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rps15-ycf1

SSC · 122231-122663 · 433 bp

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Pi 0.21

MarkerSeek score 67.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 433
variable_sites: 177
pi: 0.21
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.0	58.0	1062-1071 bp	0.5	60.5
rps15-ycf1_p2	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	925-934 bp	0.5	60.3
rps15-ycf1_p3	CCGCAGACCTCTCTGAGATA	AATAATGGGAGCCGCTTGAG	55.0	50.0	58.0	58.0	648-651 bp	0.5	60.1
rps15-ycf1_p4	AATAAGCCAGCAACCGTTGA	AATAATGGGAGCCGCTTGAG	45.0	50.0	58.1	58.0	685-688 bp	0.5	59.5
rps15-ycf1_p5	CCAGAATTTTCCGCAGACCT	AATAATGGGAGCCGCTTGAG	50.0	50.0	58.2	58.0	658-661 bp	0.444444	56.5