Hotspot

ndhF-rpl32

SSC · 110943-111990 · 1048 bp

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Pi 0.185

MarkerSeek score 73.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1048
variable_sites: 377
pi: 0.185
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CACCACCACAATGTGAGTGA	50.0	50.0	58.0	58.0	1554-1556 bp	0.5	60.0
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CACCACCACAATGTGAGTGA	45.0	50.0	58.0	58.0	1552-1554 bp	0.5	59.9
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CACCACCACAATGTGAGTGA	45.0	50.0	58.0	58.0	1553-1555 bp	0.5	59.9
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	AATAAGACTCCCCACCACCA	50.0	50.0	58.0	57.9	1566-1568 bp	0.5	59.9
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	AATAAGACTCCCCACCACCA	45.0	50.0	58.0	57.9	1564-1566 bp	0.5	59.8