Hotspot

ndhF-rpl32

SSC · 114923-115658 · 736 bp

Back to Circaea

Pi 0.0154

MarkerSeek score 68.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 736
variable_sites: 37
pi: 0.0154
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACTGGTAGTGGAATGAAAGGT	GCTGCCCAATATGCCTTTTT	42.9	45.0	56.5	57.3	836-912 bp	1.0	62.2
ndhF-rpl32_p2	AGGAACTGGTAGTGGAATGA	GCTGCCCAATATGCCTTTTT	45.0	45.0	55.5	57.3	840-916 bp	1.0	61.4
ndhF-rpl32_p3	AACTGGTAGTGGAATGAAAGGT	GCTGCCCAATATGCCTTTTT	40.9	45.0	57.3	57.3	837-913 bp	1.0	60.0
ndhF-rpl32_p4	ACTGGTAGTGGAATGAAAGGT	CTTTCAGCGCTGCCCAATAT	42.9	50.0	56.5	59.0	844-920 bp	1.0	60.0
ndhF-rpl32_p5	AGGAACTGGTAGTGGAATGAA	GCTGCCCAATATGCCTTTTT	42.9	45.0	56.3	57.3	840-916 bp	1.0	59.9