Hotspot

psaJ-rpl33

LSC · 70177-70729 · 553 bp

Back to Circaea

Pi 0.00706

MarkerSeek score 59.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 13
pi: 0.00706
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	GCATCTTTACCCTTGGCCAT	50.0	50.0	58.0	58.2	670-702 bp	1.0	83.0
psaJ-rpl33_p2	TGGCACCGGTACTAAGTACT	GCATCTTTACCCTTGGCCAT	50.0	50.0	57.8	58.2	657-689 bp	1.0	81.6
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	TTACACGGGCATCTTTACCC	50.0	50.0	58.0	57.6	678-710 bp	1.0	81.4
psaJ-rpl33_p4	ACATATCTTTCCGTGGCACC	TACACGGGCATCTTTACCCT	50.0	50.0	58.0	58.4	677-709 bp	1.0	81.3
psaJ-rpl33_p5	GTGGCACCGGTACTAAGTAC	GCATCTTTACCCTTGGCCAT	55.0	50.0	57.7	58.2	658-690 bp	1.0	81.1