Hotspot

rbcL-accD

LSC · 59435-60212 · 778 bp

Back to Circaea

Pi 0.00677

MarkerSeek score 55.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 778
variable_sites: 15
pi: 0.00677
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGCTGCTGCTTGTGAAGTA	TGTACTTAGACCACGCCTGT	45.0	50.0	58.0	58.4	907-912 bp	1.0	78.6
rbcL-accD_p2	AATTATACGCGAGGCTTGCA	TGTACTTAGACCACGCCTGT	45.0	50.0	58.1	58.4	942-947 bp	1.0	78.4
rbcL-accD_p3	ATTATACGCGAGGCTTGCAA	TGTACTTAGACCACGCCTGT	45.0	50.0	58.1	58.4	941-946 bp	1.0	78.4
rbcL-accD_p4	TATACGCGAGGCTTGCAAAT	TGTACTTAGACCACGCCTGT	45.0	50.0	58.1	58.4	939-944 bp	1.0	78.4
rbcL-accD_p5	TGCTGCTGCTTGTGAAGTAT	TGTACTTAGACCACGCCTGT	45.0	50.0	57.8	58.4	906-911 bp	1.0	77.3